Cattle on the motorway and an overturned vehicle brought traffic to a standstill on the M58.
There was a crash on the eastbound carriageway between junction five at Up Holland and six for the Orrell Interchange shortly before 9.40am on Thursday.
A Land Rover with a trailer carrying cattle overturned and two lanes of the motorway had to be closed.
Highways England said it also shut the westbound carriageway while the animals were moved to a replacement trailer.
The motorway was cleared by 11.30am.